HPLC Analysis of Water Soluble B Vitamins on Ascentis® Express RP-Amide

HPLC Analysis of Water Soluble B Vitamins on Ascentis® Express RP-Amide

Conditions

column Ascentis Express RP-Amide, 5 cm x 3.0 mm I.D., 2.7 µm particles (53916-U)
column temp. 35 °C
mobile phase [A] 20 mM potassium phosphate, dibasic, pH 6.2; [B] acetonitrile
gradient 0% B for 0.5 min, to 15% B over 0.3 min; up to 35% B in 1.2 min, 1 min equilibration at 0% B
flow rate 2.0 mL/min
pressure 5076 psi (350 bar)
sample 9 vitamins in water, 10 µg/mL for nicotinic acid and folic acid, 12 µg/mL for thiamine hydrochloride and cyanocobalamin, (20 µg/mL for pyridoxine, 30 µg/mL for riboflavin, 40 µg/mL for niacineamide, 100 µg/mL for calcium pantothenate, 120 µg/mL for biotin)
injection 5 µL
detector UV, 210 nm

Description

Analysis Note A mix of 9 water-soluble B-vitamins was separated on Ascentis Express RPA column
Categories Analytical Chromatography, Pharmaceutical Analysis, Anticonvulsant/antiepiletic/antiseizure, Vitamins and Natural products
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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