HPLC Analysis of Water-Soluble B-Vitamins on Ascentis® Express C18

HPLC Analysis of Water-Soluble B-Vitamins on Ascentis® Express C18

Conditions

column Ascentis Express C18, 5 cm x 3.0 mm I.D., 2.7 µm particles (53811-U)
column temp. 35 C
mobile phase [A] 20 mM potassium phosphate, dibasic, pH 7; [B] methanol
gradient 0.5% B for 1.3 min, 0.5-30%B over 1.7 min; to 30% B in 1.4 min, 2 min equilibration at 0.5% B
flow rate 1.0 ml/min
pressure 4130 psi (285 bar)
sample 9 vitamins in water, 10 µg/mL for nicotinic acid and folic acid, 12 µg/mL for thiamine hydrochloride and cyanocobalamin, (20 µg/mL for pyridoxine, 30 µg/mL for riboflavin, 40 µg/mL for niacineamide, 100 µg/mL for calcium pantothenate, 120 µg/mL for biotin)
detector UV, 210 nm

Description

Analysis Note A mix of 9 water-soluble B-vitamins was separated on Ascentis Express C18 column
Categories Analytical Chromatography, Pharmaceutical Analysis, Anticonvulsant/antiepiletic/antiseizure, Vitamins and Natural products
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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