HPLC Analysis of Withania Extract on Ascentis® Express Phenyl-Hexyl

HPLC Analysis of Withania Extract on Ascentis® Express Phenyl-Hexyl

Conditions

column Ascentis Express Phenyl-Hexyl, 10 cm x 2.1 mm I.D., 2.7 µm particles (53336-U)
column temp. 35 °C
mobile phase [A] water; [B] acetonitrile
gradient 20 to 100% B in 10 min; held at 100% B for 0.5 min
flow rate 0.3 mL/min
pressure 160 bar (2320 psi)
sample 20 mg/mL in water:acetonitrile (50:50); sonicate 15 minutes; filter 0.45 µm;dilute to water:acetonitrile (80:20)
injection 5 µL
detector UV, 227 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis Express Phenyl-Hexyl for the efficient separation of withania extract.
Categories Analytical Chromatography, Herbal medicines
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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