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HPLC Analysis of Yohimbe Alkaloids on Ascentis® Express F5, HILIC Mode

HPLC Analysis of Yohimbe Alkaloids on Ascentis® Express F5, HILIC Mode

Conditions

column Ascentis Express F5, 3 cm x 3.0 mm I.D., 2.7 µm particles (53574-U)
column temp. 35 C
mobile phase 2 mM ammonium acetate in 10:90 water:acetonitrile, pH to 6.0 with acetic acid
flow rate 0.5 ml/min
pressure 1100 psi (76 bar)
sample 100 ug/mL each in methanol
injection 2 µl
detector UV, 275 nm

Description

Categories Analytical Chromatography, Vitamins and Natural products
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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