HPLC Analysis of Yohimbe Extract Adulterated with Erectile Dysfunction Drugs on Ascentis® Express RP-Amide

HPLC Analysis of Yohimbe Extract Adulterated with Erectile Dysfunction Drugs on Ascentis® Express RP-Amide

Conditions

sample preparation Filter with 0.22 µm PVDF (Z227498)
sample preparation Sonicate 20 min
sample preparation Solvent Desorption
extraction Weigh approximately 100 mg of herbal supplement and add 10 mL of 50:50, water:ethanol
column Ascentis Express RP-Amide, 10 cm x 3.0 mm I.D., 2.7 µm particles (53918-U)
column temp. 35 °C
mobile phase [A] water with 0.1% formic acid; [B] acetonitrile with 0.1% formic acid
gradient 5% B held for 0.5 min; to 100% B in 9.5 min; 100% B held for 2 min
flow rate 0.4 mL/min
pressure 870 psi60 bar (60 bar)
sample Extract 10mg/mL before filtration, adulteration 20µg/mL in 50:50, water:ethanol
injection 2 µL
detector UV, 250 nm

Description

Categories Analytical Chromatography, ED Drugs
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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