HPLC Analysis of Z-Drugs on Ascentis® Express ES-Cyano 2.0 μm

HPLC Analysis of Z-Drugs on Ascentis® Express ES-Cyano 2.0 μm

Conditions

column Ascentis Express ES-Cyano, 10 cm x 3.0 mm I.D., 2.0 µm particles (51732-U)
column temp. 30 °C
mobile phase [A] 10 mM ammonium formate pH 3.0 with formic acid; [B] acetonitrile; (70:30, A:B)
flow rate 0.6 mL/min
pressure 5405 psi (373 bar)
sample 10 µg/mL in 75:25, water:methanol
injection 2 µL
detector UV, 254 nm

Description

Analysis Note An rapid method for the siµLtaneous determination of the Z-drugs or sleep aids: zopiclone, zolpidem, and zaleplon is presented here. The need for greater analytical capacity and throughput for the analysis of sleep aid medicines (Z-drugs) in forensic toxicology laboratories can be met by the use of fast Ascentis Express 2.0 micron Fused Core UHPLC Columns.
Featured Industry Clinical
Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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