HPLC Separation of an Oligonucleotide and Its Degradation Fragments on Proteomix® SAX-NP5

HPLC Separation of an Oligonucleotide and Its Degradation Fragments on Proteomix® SAX-NP5

Conditions

column Proteomix SAX-NP5, 15 cm x 4.6 mm I.D., 5 µm particles (Z777228)
mobile phase (A) 25 mM Tris, 1 mM EDTA 10% acetonitrile (8.0 pH); (B) A + 1.0 M NaCl
gradient 0 to 75% B in 50 min
flow rate 0.5 mL/min
sample Oligonucleotide (MW 12196) and its degradation fragments

Description

Categories Analytical Chromatography, Nucleic Acids
Featured Industry Forensics and Toxicology
Life Science and Biopharma
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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