HPLC Analysis of Nucleobases using TSKgel® SuperSW mAb HTP column in HILIC mode

HPLC Analysis of Nucleobases using TSKgel® SuperSW mAb HTP column in HILIC mode

Conditions

column TSKgel Amide-80 10 cm x 2 mm I.D., 5 µm particles (819695)
column TSKgel SuperSW mAb HTP 15 cm x 4.6 mm I.D., 4 µm particles (822855)
column temp. Ambient
mobile phase SEC conditions: 100 mmol/L phosphate/100 mmol/L sodium sulfate, pH 6.7 + 0.05% NaN3 (SEC mode)
mobile phase HILIC mode: [A]: acetonitrile (HILIC mode); [B]: 15 mmol/L ammonium bicarbonate, pH 7.4 (HILIC mode)
gradient Isocratic
flow rate 0.4 mL/min
sample uracil (1.5 mg/mL), adenine (1.5 mg/mL), cytosine (1.5 mg/mL), cytidine (1.5 mg/mL)
injection 1 µL
detector UV, 280 nm

Description

Categories Analytical Chromatography, Nucleic Acids
Featured Industry Life Science and Biopharma
Legal Information TSKgel is a registered trademark of Tosoh Corporation
suitability application for HPLC

Materials

     
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