Hyper-Fast Separations on Ascentis® Express 2.7 μm at Half the Pressures of sub-2 μm Columns

Hyper-Fast Separations on Ascentis® Express 2.7 μm at Half the Pressures of sub-2 μm Columns

Conditions

column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 µm particles and sub-2 µm particle column, 5 cm x 2.1 mm I.D. (53823-U)
column temp. ambient
mobile phase [A] water; [B] acetonitrile; (55:45 or 54:46, A:B)
flow rate 0.2 ml/min
injection 1 µl
detector UV, 200 nm

Description

Categories Analytical Chromatography, Hormones
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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