LC/MS Analysis of 25-Hydroxyvitam D3 and 25-Hydroxyvitamin D2 on Ascentis® Express C18

LC/MS Analysis of 25-Hydroxyvitam D3 and 25-Hydroxyvitamin D2 on Ascentis® Express C18

Conditions

column Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 µm particles (53822-U)
column temp. 35 °C
mobile phase [A] 10mM ammonium formate in water; [B] 10mM ammonium formate in acetonitrile; (32:68, A:B)
flow rate 0.4 mL/min
pressure 1711 psi (118 bar)
sample 10 µg/mL in 25:75, water:methanol
injection 2 µL
detector ESI(+)

Description

Categories Analytical Chromatography, Clinical, vitamins, Vitamins and Natural products
Featured Industry Clinical
Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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