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LC/MS Analysis of Amphetamine Enantiomers on Astec CHIROBIOTIC® V2

LC/MS Analysis of Amphetamine Enantiomers on Astec CHIROBIOTIC® V2

Conditions

column Astec CHIROBIOTIC V2, 15 cm x 4.6 mm I.D., 5 µm particles (15023AST)
column temp. 20 C
mobile phase [A] methanol; [B] water; [C] acetic acid; [D] ammonium hydroxide; (95:5:0.1:0.02, A:B:C:D
flow rate 1 ml/min
pressure 1220 psi (84 bar)
sample 50 ng/mL each enantiomer in methanol
injection 2 µl
detector MS, ESI(+),SIR m/z 150.1

Description

Analysis Note Astec CHIROBIOTIC columns are unique in their ability to operate in MS-friendly mobile phases, and with polar analytes. Demonstrated here is CHIROBIOTIC V2 for the resolution of the enantiomers of amphetamine. High purity solvents provided robust operation, and Cerilliant CRMs provided reliable quantification.
Categories Analytical Chromatography, Forensics and Toxicology, Drugs of Abuse, Enantiomers, enantiomers, drugs of abuse
Featured Industry Forensics and Toxicology
Legal Information CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
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