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LC-MS Analysis of Androsterone Steroid Isomers on Ascentis® Express RP-Amide

LC-MS Analysis of Androsterone Steroid Isomers on Ascentis® Express RP-Amide

Conditions

column Ascentis Express RP-Amide, 5cm x 3.0mm I.D., 2.7mum particles
column temp. 35 C
mobile phase [A] water with 0.1% formic acid; [B] acetonitrile with 0.1% formic acid 50:50 (A:B)
flow rate 0.7 ml/min
pressure 3900 psi (269 bar)
sample 500 ng/mL in mobile phase
injection 2 µl
detector MS, SIM, ESI+

Description

Analysis Note The application deonstrates the suitability of Ascentis Express RP-Amide for the separation of androsterone and trans-androsterone. The polar embedded group provides good selectivity for these compounds based interaction between the amide carbonyl and the steroid phenol group.
Categories Analytical Chromatography, Steroids
Featured Industry Clinical
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
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