LC/MS Analysis of Anesthetics on Ascentis® Express F5

LC/MS Analysis of Anesthetics on Ascentis® Express F5

Conditions

column Ascentis Express F5, 10 cm x 4.6 mm I.D., 2.7 µm particles (53590-U)
column temp. 35 °C
mobile phase [A] 10 mM ammonium acetate, pH 4.0 with acetic acid; [B] acetonitrile; (20:80, A:B)
flow rate 1.0 mL/min
pressure 2320 psi (160 bar)
sample 10 µg/mL in methanol
injection 2 µL
detector MS, ESI(+), SIR, m/z 235, 236, 247

Description

Categories Analytical Chromatography, Anesthetics
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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