LC/MS Analysis of Angiotensins on Discovery® BIO Wide Pore C18

LC/MS Analysis of Angiotensins on Discovery® BIO Wide Pore C18

Conditions

column Discovery BIO Wide Pore C18, 10 cm x 0.32 mm I.D., 3 µm particles (65527-U)
column temp. ambient
mobile phase [A] 65:35, (10mM ammonium acetate, pH 7):(50% acetonitrile in 20mM ammonium acetate, pH 7); [B] 25:75, (10mM ammonium acetate, pH 7):(50% acetonitrile in 20mM ammonium acetate, pH 7)
gradient 0-100% B in 12.5 min
flow rate 6 µL/min
injection 50 pmol angiotensin I, II and III in water
detector +ESI mode

Description

Categories Analytical Chromatography, Peptides
Featured Industry Pharmaceutical (small molecule)
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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