LC/MS Analysis of Annato (or Roucou or Achiote) on Chromolith® FastGradient RP-18 endcapped

LC/MS Analysis of Annato (or Roucou or Achiote) on Chromolith® FastGradient RP-18 endcapped

Conditions

column Chromolith FastGradient RP-18, 5 cm x 2.0 mm I.D, 2 µm particles (1.52007.0001)
column temp. 25 C
mobile phase [A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile
gradient 55 to 64% B in 0.5 min, to 70% B in 3 min, to 90% B in 0.5 min, held at 90% B for 2 min, to 55% B in 0.5 min, held at 55% for 2.5 min
flow rate 0.4 ml/min
pressure 562-317 psi (39 - 22 bar)
injection 10 µl
detector ESI-MS(+) (m/z range 378-396), BPC

Description

Categories Analytical Chromatography, Dyes
Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes Sample Prep: Dissolve 2.2 mg/mL cis-norbixin and 0.12 mg/mL cis-bixin in diluent. After dissolution of the analytes the solution was heated for 5.5 hr at 75 °C under reflux to speed up transformation of cis- to trans-isomers and thereafter diluted 1:10 with acetonitrile and water (50:50 v/v).
suitability application for LC-MS

Materials

     
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