Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

LC/MS Analysis of Antifungals on Ascentis® Express Phenyl-Hexyl

LC/MS Analysis of Antifungals on Ascentis® Express Phenyl-Hexyl

Conditions

column Ascentis Express Phenyl-Hexyl, 10 cm x 2.1 mm I.D., 2.7 µm particles (53336-U)
column temp. 35 C
gradient [A] 10 mM ammonium acetate; [B] acetonitrile
gradient 2% B for 1 min; to 100% B in 10 min; held at 100% B for 1 min
flow rate 0.3 ml/min
pressure 2930 psi (202 bar)
sample Antifungals, 1-10 µg/mL in 99:1, water:methanol
injection 0.5 µl
detector MS, ESI+, XIC

Description

Categories Analytical Chromatography, Antifungals
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links