LC/MS Analysis of Aromatic Amines in Aqueous Extracts on SeQuant® ZIC-HILIC

LC/MS Analysis of Aromatic Amines in Aqueous Extracts on SeQuant® ZIC-HILIC

Conditions

column Sequant ZIC-HILIC, PEEK, 150 X 1.0 mm, 3.5 um, 200 Å (1.51480.0001)
mobile phase [A] acetonitrile; [B] 0.01% (v/v) acetic acid
gradient 30% to 50% B in 10 min
flow rate 0.5 mL/min
detector LC–MS/MS operated in positive electrospray ionisation (ESI) mode
injection 5 μL, 1μL
sample 15 μL focusing liquid (acetonitrile/water (95/5, v/v) containing aromatic amines
(0.125 μg/mL) and deuterated analogues (0.25 μg/mL)

Description

Featured Industry Chemicals and Industrial Polymers
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suitability application for LC-MS
other notes cone voltage of 25 V, capillary voltage of 4.65 kV,collision energy of 15 eV for TDA and 25 eV for MDA, desolvation temperature of 200 ◦C andion source temperature of 130 °C. MRM performed for [M+H]+ →[M−17]+for TDA and [M+H]+ →[M−93]+ for MDA with a dwell time of 0.2 sec

Materials

     
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