LC/MS Analysis of Conjugated Bile Acids on Ascentis® Express RP-Amide

LC/MS Analysis of Conjugated Bile Acids on Ascentis® Express RP-Amide

Conditions

column Ascentis Express RP-Amide, 5 cm x 4.6 mm I.D., 2.7 µm particles (53922-U)
column temp. 35 °C
mobile phase [A] 10 mM ammonium acetate, pH 3 with acetic acid; [B] acetonitrile
gradient 25 to 40% B in 10 min
flow rate 1.0 mL/min (postcolumn split to the MS)
sample 10 mg/L in mobile phase A
injection 5 µL
detector MS, ESI(-), SIR

Description

Categories Analytical Chromatography, Clinical, organic acids, Organic Acids
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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