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LC/MS Analysis of Ethanol Metabolites Ethyl Sulfate and Ethyl-β-D-Glucuronide on Ascentis® Express OH5

LC/MS Analysis of Ethanol Metabolites Ethyl Sulfate and Ethyl-β-D-Glucuronide on Ascentis® Express OH5

Conditions

column Ascentis Express OH5, 10 cm x 2.1 mm I.D., 2.7 µm particles (53757-U)
column temp. 35 C
mobile phase 5 mM ammonium formate in 95:5, acetonitrile:water, adjusted to pH 4.0 with formic acid
flow rate 0.4 ml/min
pressure 1200 psi (83 bar)
sample ethyl sulfate (2 µg/mL), ethyl-beta-D-glucuronide (20 µg/mL) in 98:2, acetonitrile:methanol
injection 10 µl
detector MS, ESI(-), combined XIC, 125.0 m/z, 221.1 m/z

Description

Analysis Note The compounds ethyl sulfate and ethyl-β-D-glucuronide are metabolites of ethanol. Their analysis is of clinical interest as biomarkers or indicators of alcohol-induced liver disease (ALD). The HPLC analysis of these compounds using reversed-phase mode on C18 columns suffers from poor retention due to the compounds′ polar nature. However, by using HILIC mode on an Ascentis Express OH5 column, both retention and MS-compatibility are improved. Cerilliant CRMs provided reliable quantification.
Categories Analytical Chromatography, Clinical, Forensics and Toxicology, alcohols, drugs of abuse, Alcohols, Drugs of Abuse
Featured Industry Clinical
Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
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