LC/MS Analysis of Fentanyl and Related Compounds in Urine on Ascentis® Express Biphenyl

LC/MS Analysis of Fentanyl and Related Compounds in Urine on Ascentis® Express Biphenyl

Conditions

column Ascentis Express Biphenol 5 cm x 2.1mm I.D., 2.7mum particles (64057-U)
column temp. 30 °C
mobile phase [A] 0.1% formic acid in water; [B] 0.1% formic acid in methanol
gradient 40 to 90% B in 1.5 min; held at 90% B for 1 min
flow rate 0.6 mL/min
pressure 4206 psi (290 bar)
injection 0.5 µL
detector MS, ESI(+), SIM, m/z 233, 323, 337, 387

Description

Categories Analytical Chromatography, Drugs of Abuse, Opiate
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes Sample prep: synthetic urine spiked with norfentanyl (800 ng/mL), acetyl fentanyl (400 ng/mL), fentanyl (800 ng/mL), sufentanil(800 ng/mL), then diluted 1 to 9 in water
suitability application for LC-MS

Materials

     
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