LC/MS Analysis of Fluoroamphetamine Drugs of Abuse in Urine on Ascentis® Express HILIC after SPE using Supel™-Select SCX

LC/MS Analysis of Fluoroamphetamine Drugs of Abuse in Urine on Ascentis® Express HILIC after SPE using Supel™-Select SCX

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix urine, 1 mL
SPE tube/cartridge Supel-Select SCX SPE 96-well Plate, 30 mg/well (575664-U)
condition 1 mL methanol, then 1 mL 0.1% formic acid in water
washing 2 x 1 mL 10% methanol in water, 2 x 1 mL 1% formic acid in methanol
elution 2 mL 5% ammonium hydroxide in acetonitrile
eluate post-treatment evaporate to 500 µl with nitrogen at room temperature, reconstitute in 250 µl acetonitrile
column Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 µm particles (53939-U)
column temp. 35 C
mobile phase 5 mM ammonium formate in acetonitrile:water (90:10, v/v)
flow rate 0.4 ml/min
pressure 1570 psi (108 bar)
injection 2 µl
detector TOF/MS

Description

Analysis Note The fluoroamphetamines (FA) are a group of "designer" stiµlant drugs in the amphetamine family. Four members of the FA family are shown here isolated from urine using Supel-Select SCX SPE and separated in under 3 minutes on an Ascentis Express HILIC column. Cerilliant CRMs were used to ensure reliable MS identification and quantification.
Categories Analytical Chromatography, SPE Applications, Drugs of Abuse, Drugs of abuse, Pharmaceutical & Clinical applications
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, Supel is a trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS, application for SPE

Materials

     
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