LC/MS Analysis of Fourteen Acyl-L-Carnitines on Ascentis® Express OH5

LC/MS Analysis of Fourteen Acyl-L-Carnitines on Ascentis® Express OH5

Conditions

column Ascentis Express OH5, 15 cm x 2.1 mm I.D., 2.7 µm particles (53764-U)
column temp. 30 °C
mobile phase [A] 50 mM ammonium formate, pH 3; [B] acetonitrile
gradient 2% A for 0.2 min, to 26% A in 14.8 min; to 50% A in 0.1 min; held at 50% A for 4.9 min; to 2% A in 0.1 min
flow rate 0.4 mL/min
pressure 3640 psi (251 bar)
sample each compound, 0.1 ng on column, except carnitine, 0.2 ng on column
injection 0.1 µL
detector MS, ESI(+), 120-500 m/z

Description

Application Carnitine plays an important role in fatty acid metabolism. A prerequisite for studies on the wide variety of acyl-L-carnitines and their function is the availability of acyl-L-carnitine standards and a reliable and sensitive analytical methodology. LC/MS using hydrophilic interaction liquid chromatography (HILIC) on an Ascentis Express OH5 column is shown here for the separation of free carnitine and acylcarnitines without derivatization.
Categories Analytical Chromatography, Amino Acids
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
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