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LC/MS Analysis of Isoflavones on Chromolith® FastGradient RP-18 endcapped

LC/MS Analysis of Isoflavones on Chromolith® FastGradient RP-18 endcapped

Conditions

column Chromolith FastGradient RP-18, 5 cm x 2.0 mm I.D., 2 µm particles (1.52007.0001)
column temp. 25 C
mobile phase [A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile
gradient 5 to 15% B in 1.5 min, to 95% B in 4 min, held at 95% B for 1 min, to 5% B in 1 min, held at 5% for 1.5 min
flow rate 0.5 ml/min
pressure 821-317 psi (57 - 22 bar)
sample genistein 10 ppb, daidzein 10 ppb, glycitin 10 ppb, puerarin 20 ppb in methanol and water (50:50 v/v)
injection 1 µl
detector ESI-MS(+) (m/z range 100-600), overlay of four EICs (m/z 271.0, 255.0, 447.1, 417.1)

Description

Categories Analytical Chromatography, Dyes
Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for LC-MS

Materials

     
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