LC/MS Analysis of Lactic Acid Enantiomers in Urine on Astec® CHIROBIOTIC® R

LC/MS Analysis of Lactic Acid Enantiomers in Urine on Astec® CHIROBIOTIC® R

Conditions

column Astec CHIROBIOTIC R, 15 cm x 2.1 mm I.D., 5 µm particles (13019AST)
column temp. 4 °C
mobile phase [A] 33.3 mM ammonium acetate; [B] acetonitrile; (15:85, A:B)
flow rate 0.7 mL/min
sample Urine extracts in 5% (v/v) acetic acid, 10% (v/v) H2O and 85% (v/v) acetonitrile
injection 20 µL
detector (-)ESI

Description

Analysis Note Chromatogram supplied courtesy of H. Henry and associates at the Clinical Chemistry Laboratory, Centre Hospitalier Universitaire Vaudois, University of Lausanne, CH-1011, Switzerland. View the related full-length contributed article in Reporter 30.1.
Categories Analytical Chromatography, Chiral Applications, Clinical, Clinical, Forensic & Toxicology, Carboxylic acids, carboxylic acids, Biomarkers
Featured Industry Clinical
Legal Information CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC, Astec is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes Reference: Biomed. Chromatogr. 2011 "Sensitive determination of D-lactic acid and L-lactic acid in urine by high-performance liquid chromatography–tandem mass spectrometry" H. Henry, N. Marmy Conus, P. Steenhout, A. Béguin and O. Boulat, Published online in Wiley Online Library, wileyonlinelibrary.com/journal/bmc.
suitability application for HPLC

Materials

     
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