LC/MS Analysis of β-Lactoglobulin Tryptic Digest on a Discovery® BIO Wide Pore C18 Capillary Column

LC/MS Analysis of β-Lactoglobulin Tryptic Digest on a Discovery® BIO Wide Pore C18 Capillary Column

Conditions

column Discovery BIO Wide Pore C18, 15 cm x 0.5 mm I.D., 5 µm particles (65519-U)
column temp. 30 °C
mobile phase [A] 0.1% TFA in water; [B] 0.1% TFA in acetonitrile
gradient 5-40% B in 70 min
flow rate 14 µL/min
injection 500 pmol (5µl) beta-Lactoglobulin tryptic digest in 50 mM ammonium bicarbonate
detector +ESI mode

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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