LC/MS Analysis of Meropenem in Plasma on Titan™ Silica 1.9 μm

LC/MS Analysis of Meropenem in Plasma on Titan™ Silica 1.9 μm

Conditions

sample preparation 250 µl of rat plasma spiked with 500 ng/mL meropenem, protein precipitation by addtion of 750 µl of 1% formic acid in acetonitrile, (centrifuge 10,000 rcf for 5 min, dilute 3:1 with 1% formic acid in acetonitrile prior to injection)
column Titan Silica, 10 cm x 3.0 mm I.D., 1.9 µm particles (581536-U)
column temp. 35 C
mobile phase [A] acetonitrile; [B] 100 mM ammonium formate; (75:25, A:B); pH to 5.0 with formic acid after addition of organic
flow rate 0.5 ml/min
pressure 2560 psi (177 bar)
injection 2 µl
detector MS ESI+, SIR, 384.2 m/z

Description

Categories Analytical Chromatography, Antibiotics, Antimicrobials
Featured Industry Pharmaceutical (small molecule)
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
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