LC/MS Analysis of Methamphetamine Enantiomers on Astec® CHIROBIOTIC® V2 in Urine after SPE using Supel™-Select SCX

LC/MS Analysis of Methamphetamine Enantiomers on Astec® CHIROBIOTIC® V2 in Urine after SPE using Supel™-Select SCX

Conditions

sample preparation SPE (Solid Phase Microextraction)
sample/matrix 1 mL of synthetic urine spiked with 25 ng/mL
extraction process acidified to 3-4 pH with formic acid
SPE well plate Supel-Select SCX SPE 96-well Plate, 30 mg/well (575664-U)
condition 1 mL 1% formic acid in acetonitrile, then 1 mL water
washing 2 mL water, then 1 mL 25% methanol
elution 1 mL 10% ammonium hydroxide in acetonitrile
eluate post-treatment evaporate to dryness under nitrogen at 40 °C, reconstitute in 1 mL mobile phase
column Astec CHIROBIOTIC V2, 15 cm x 4.6 mm I.D., 5 µm particles (15023AST)
column temp. 20 °C
mobile phase [A] methanol; [B] water; [C] acetic acid; [D] ammonium hydroxide; (95:5:0.1:0.02, A:B:C:D)
flow rate 1.0 mL/min
pressure 1221 psi (84 bar)
sample 1.0 mLurine
injection 2 µL
detector MS, ESI(+),SIR m/z 150.1

Description

Categories Analytical Chromatography, Forensics and Toxicology, SPE Applications, Drugs of abuse, enantiomers, drugs of abuse, Drugs of Abuse, Enantiomers
Featured Industry Forensics and Toxicology
Legal Information Supel is a trademark of Sigma-Aldrich Co. LLC, CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC, Astec is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes sample pretreatment: acidified to 3-4 pH with formic acid
suitability application for LC-MS, application for SPE

Materials

     
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