LC/MS Analysis of Nicotine and Metabolites on Kromasil® Eternity™ C18 (Analyte Set 1)

LC/MS Analysis of Nicotine and Metabolites on Kromasil® Eternity™ C18 (Analyte Set 1)

Conditions

column Kromasil Eternity C18, 10 cm x 3.0 mm I.D., 2.5 µm particles (K0897111)
column temp. 45 °C
mobile phase [A] 10 mM ammonium acetate in water:methanol (95:5); [B] 10 mM ammonium acetate in water:methanol (10:90)
gradient 0% B held for 1 min; to 100% B in 8.5 min;100% B held for 1 min
flow rate 0.4 mL/min
pressure 332 psi (23 bar)
sample 100 ng/mL in mobile phase A
injection 5 µL
detector MS, ESI(+) TOF, EIC

Description

Analysis Note Nicotine and metabolites are shown here separated on a Kromasil Eternity C18 column. High purity LC/MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Clinical, Amines, amines
Featured Industry Clinical
Legal Information Kromasil is a registered trademark of EKA Chemicals AB, Eternity is a trademark of EKA Chemicals AB
suitability application for HPLC

Materials

     
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