LC/MS Analysis of Pain Management Opioids on Ascentis® Express Biphenyl

LC/MS Analysis of Pain Management Opioids on Ascentis® Express Biphenyl

Conditions

column Ascentis Express Biphenyl, 10 cm x 2.1 mm I.D., 2.7 µm particles (64065-U)
column temp. 30 °C
mobile phase [A] water with 0.1% formic acid; [B] methanol with 0.1% formic acid
gradient 10 to 20% B in 3 min; to 100% B in 3.5 min
flow rate 0.3 mL/min
pressure 2930 psi (202 bar)
sample Pain Management µLti-Component Opiate Mixture-13 (P-071) dilute to 30-300 ng/mL in 99:1, water:methanol
injection 2 µL
detector MS-TOF, ESI(+), XIC

Description

Categories Analytical Chromatography, Opiate, Analgesics
Featured Industry Clinical
Forensics and Toxicology
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
Related Links