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LC/MS Analysis of Peptides from a Tryptic Digest of Cytochrome C on SeQuant ZIC-HILIC

LC/MS Analysis of Peptides from a Tryptic Digest of Cytochrome C on SeQuant ZIC-HILIC

Conditions

column SeQuant ZIC-HILIC, PEEK, 150 x 0.3 mm, 5 µm, 200 Ã… (1.50481.0001)
mobile phase [A] 0.25% (v/v) formic acid; [B] 0.25% (v/v) formic acid in acetonitrile
flow rate 5 µL/min
sample 10 pmole digested sample in mobile phase
detector MicroMass Ultima-QTOF, Needle voltage: 3.1 kV, Cone voltage: 45V (collision energy: 10V; Scan range: m/z 400-1800; 1.5 s cycle time)

Description

Featured Industry Life Science and Biopharma
suitability application for LC-MS

Materials

     
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