LC/MS Analysis of Peptides on Discovery® BIO Wide Pore C18, Effect of TFA Concentration on MS Signal

LC/MS Analysis of Peptides on Discovery® BIO Wide Pore C18, Effect of TFA Concentration on MS Signal

Conditions

column Discovery® BIO Wide Pore C18, 15 cm x 2.1 mm I.D., 3 µm particles (567202-U)
column temp. ambient
mobile phase [A]: aqueous component (25 mM formic acid, 0.01% TFA or 0.1% TFA); [B] (50:50) water:acetonitrile containing same ionic additives as aq. component
flow rate 0.20 mL/min
sample RP Peptide Performance Standard (Alberta Peptide Inst., Cat. No. RPS-P0010)
injection 1-3 µL
detector (+)ESI

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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