Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

LC/MS Analysis of Phosphatidylethanol Metabolites on Titan™ PFP 1.9 μm

LC/MS Analysis of Phosphatidylethanol Metabolites on Titan™ PFP 1.9 μm

Conditions

column Titan PFP, 5 cm x 2.1 mm I.D., 1.9 µm particles (569677-U)
column temp. 50 C
mobile phase 5 mM ammonium formate in [A] water; [B] methanol; [C] acetonitrile; (20:40:40, A:B:C)
flow rate 0.5 ml/min
pressure 3680 psi (254 bar)
sample 500 ng/mL in 50:50, water:methanol
injection 2 µl
detector MS, ESI-, combined SIR, 699.7, 701.7 m/z

Description

Analysis Note Phosphatidylethanol and its metabolites in blood are markers of ethanol consumption. Shown here is the rapid resolution of the metabolites on a Titan PFP column.
Categories Analytical Chromatography, Drugs of Abuse
Featured Industry Forensics and Toxicology
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
Related Links