LC/MS Analysis of Triclosan (Irgasan) in Waste Water on Ascentis® Express C18 after SPE using Supel™-Select HLB

LC/MS Analysis of Triclosan (Irgasan) in Waste Water on Ascentis® Express C18 after SPE using Supel™-Select HLB

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix waste-water treatment plant effluent
SPE tube/cartridge Supel Select HLB 60 mg/ 3 mL (54182-U)
condition 3 mL methanol, 3 mL distilled water, 3 mL 0.01 M HCl
sample addition 50 mL
washing wash using 10 mL distilled water, dry for 5 minutes under vacuum
elution 3 ml acetonitrile:methanol (1:1)
eluate post-treatment evaporated to dryness and reconstituted into the HPLC mobile phase
column Ascentis Express C18, 15 cm x 2.1 mm I.D., 2.7 µm particles (53825-U)
column temp. 30 °C
mobile phase [A] methanol; [B] acetonitrile; [C] 0.1% formic acid; (40:40:20, A:B:C)
flow rate 0.15 mL/min
pressure 1885 psi (130 bar)
injection 10 µL
detector ESI(-), MRM, 286.8/35.0

Description

Analysis Note Triclosan is one of the most prevalent antibacterial compounds added to many consumer products. It ends up in surface and ground waters from municipal waste water treatment plants, among other routes. The compound is under scrutiny because of its link to a wide range of health effects, including skin irritation and endocrine disruption, its impact on fragile aquatic biosystems, and its promotion of antibiotic resistance. Analysis of triclosan in waste water plant effluent using Supel-Select HLB SPE cleanup and LC/MS/MS using Ascentis Express 2.7 μm C18 column is shown here.
Categories Analytical Chromatography, SPE Applications, Antifungals, Antibiotics/antimicrobials
Featured Industry Environmental
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, Supel is a trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
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