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LC/MS/MS Analysis of Drugs of Abuse: Generic Screening on Ascentis® Express F5

LC/MS/MS Analysis of Drugs of Abuse: Generic Screening on Ascentis® Express F5

Conditions

column Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 µm particles (53569-U)
column temp. 35 C
mobile phase [A] 5 mM ammonium formate water pH 4.0 formic acid; [B] 5 mM ammonium formate (95:5 acetonitrile:water)
gradient 20% B for 0.5 min; to % B in 5.5 min; held at 100% B for 3 min
flow rate 0.3 ml/min
pressure 2538 psi (175 bar)
sample each compound 1000 ng/mL in 40:60 (water:methanol)
injection 1 µl
detector MS, ESI(+), TIC, m/z 100-1000

Description

Analysis Note Shown here is a method to rapidly screen a sample for a variety of abused substances using an Ascentis Expres F5 column. The highest quality LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Forensics and Toxicology, drugs of abuse, Drugs of Abuse
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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