LC/MS/MS Analysis of Epinephrine, Metanephrine and Metabolites on Ascentis® Express OH5

LC/MS/MS Analysis of Epinephrine, Metanephrine and Metabolites on Ascentis® Express OH5

Conditions

column Ascentis Express OH5, 10 cm x 2.1 mm I.D., 2.7 µm particles (53757-U)
column temp. 35 °C
mobile phase [A] 5 mM ammonium formate; [B] 5 mM ammonium  formate in acetonitrile; (15:85, A:B)
flow rate 0.3 mL/min
pressure 1189 psi (82 bar)
sample 300 ng/mL each in acetonitrile
injection 1 µL
detector MS ESI(+), 100-1000 m/z

Description

Analysis Note HILIC mode on Ascentis Express OH5 provided rapid separation of neurotransmitters in highly MS-compatible mobile phases. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Clinical, amines, Amines
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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