LC/MS/MS Analysis of Polymeric Isocyanates as Their MDI-DBA Derivatives on Ascentis® Express C18 after Collection using ASSET™ EZ4-NCO Dry Sampler

LC/MS/MS Analysis of Polymeric Isocyanates as Their MDI-DBA Derivatives on Ascentis® Express C18 after Collection using ASSET™ EZ4-NCO Dry Sampler

Conditions

sample preparation Solvent Desorption
sample/matrix ambient air
adsorbent tube ASSET EZ4-NCO Dry Sampler (5028-U)
extraction Contact Sigma-Aldrich Technical Services for extraction and full analytical protocol.
column Ascentis Express C18, 5 cm x 2.1 mm I.D., 2 µm particles (50811-U)
column temp. 35 °C
mobile phase [A] 95:5 (0.05% formic acid in water):(0.05% formic acid in acetonitrile); [B] 95:5 (0.05% formic acid in acetonitrile):(0.05% formic acid in water)
gradient 60% to 80% B in 2 min, held for 3 min, to 100% B in 0.1 min, held for 9.9 min, to 60% B in 0.1 min, held for 3.9 min
flow rate 0.4 mL/min
pressure 3626 psi (250 bar)
sample ASSET sampler filter spike, MDI monomer (7.37 µg/mL), MDI 3-ring oligomer (0.284 µg/mL), MDI 4-ring oligomer (0.912 µg/mL)
injection 2.0 µL
detector MS/MS, ESI(+), MRM

Description

Analysis Note This application demonstrates the siµLtaneous analysis of dibutylamine derivatized isocyanate MDI monomer and oligomers.
Categories Analytical Chromatography, Air Monitoring Applications, Isocyanates
Featured Industry Industrial Hygiene
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, Asset is a trademark of Sigma-Aldrich Co. LLC
Other Notes ASSET filters were spiked at various concentrations prior to extraction.
suitability application for LC-MS

Materials

     
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