LC/MS/MS Analysis of Spice Cannabinoids in Plasma on Ascentis® Express F5 after Solid Phase Extraction (SPE) using HybridSPE®-Phospholipid

LC/MS/MS Analysis of Spice Cannabinoids in Plasma on Ascentis® Express F5 after Solid Phase Extraction (SPE) using HybridSPE®-Phospholipid

Conditions

sample/matrix rabbit plasma, unfiltered K2-EDTA spiked with Spice cannabinoids (5 ng/mL each)
SPE well plate HybridSPE-Phospholipid, 96-well plate, 50 mg bed wt., 2 mL well vol (575656-U)
sample addition to each well add 100 µL plasma, followed by a 300 µL of 1% formic acid in acetonitrile, agitate on orbital shaker for 2 minutes
elution attach collection plate and apply vacuum at 10" Hg for 4 minutes
column Ascentis Express F5, 5 cm x 2.1 mm I.D., 2.7 µm particles (53567-U)
column temp. 35 °C
mobile phase [A] 10 mM ammonium formate in water, pH 6.8 (unadjusted); [B] acetonitrile; (50:50; A:B)
flow rate 0.3 mL/min
pressure 1296 psi (89 bar)
injection 2 µL
detector MS, ESI(+), MRM, m/z 344/155 (JWH-073 metabolite), 385/155 (JWH-200), 336/121 (JWH-250), 328/155 (JWH-073), and 342/155 (JWH-018)

Description

Analysis Note Synthetic cannabinoids (Spice) are a relatively new type of designer drug used as a pseudo-legal means to get a cannabis-type high. New synthetic cannabinoids are continually being introduced as suppliers tweak the molecular structures. The ability to rapidly and reliably identify the continually changing population of these compounds in the blood or urine suspected users is a significant analytical challenge facing forensic chemists. A four prong approach using column selectivity, high purity solvents, effective sample prep, and reference standards, was used to develop a method to rapidly isolate and identify Spice cannabinoids from plasma. The Ascentis Express F5 Column gave the necessary resolution, and the highest grade LC-MS solvents and additives gave adduct-free response for maxiµm sensitivity. Sample prep employing the HybridSPE-Phospholipid was rapid and effective and the Cerilliant reference standards enabled confident identification.
Categories Analytical Chromatography, Forensics and Toxicology, SPE Applications, Drugs of Abuse, drugs of abuse, Drugs of abuse
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, HybridSPE is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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