LC/MS/MS Analysis of Testosterone and Metabolites on Ascentis® Express C18

LC/MS/MS Analysis of Testosterone and Metabolites on Ascentis® Express C18

Conditions

column Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 µm particles (53822-U)
column temp. 35 C
mobile phase [A] 5 mM ammonium formate water pH 4.0 formic acid; [B] 0.1% formic acid acetonitrile
gradient 10 to 70% B in 5 min; held at 70% B for 3 min
flow rate 0.6 ml/min
pressure 3698 psi (255 bar)
sample 1000 ng/mL in 40:60 (water:methanol)
injection 5 µl
detector MS, ESI(+), TIC, m/z 100-1000

Description

Analysis Note An Ascentis Expres F5 column provided rapid, baseline resolution of testosterone and metabolites. The highest quality LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Clinical, Steroids, steroids
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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