LC/MS/MS Analysis of Vitamins B1 and B6 on Ascentis® Express OH5

LC/MS/MS Analysis of Vitamins B1 and B6 on Ascentis® Express OH5

Conditions

column Ascentis Express  OH5, 10 cm x 2.1 mm I.D., 2.7 µm particles (53757-U)
column temp. 30 °C
mobile phase [A] water with 0.1% formic acid/10 mM ammonium formate; [B] acetonitrile with 0.1% formic acid/10 mM ammonium formate; (A:B, 15:85)
flow rate 0.2 mL/min
pressure 1150 psi (79 bar)
sample 10 ng/mL in mobile phase
injection 1 µL
detector MS, ESI(+), MRM mode

Description

Analysis Note The present work establishes an HILIC LC/MS/MS method for analysis of water soluble vitamins B1 and B6 on an Ascentis® Express OH5 column. LC/MS grade solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation.
Categories Analytical Chromatography, Immunosuppressants
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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