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LC/MS/MS Analysis of Warfarin™ Enantiomers in Plasma on Astec® CHIROBIOTIC® V after SPE using HybridSPE®-Phospholipid

LC/MS/MS Analysis of Warfarin™ Enantiomers in Plasma on Astec® CHIROBIOTIC® V after SPE using HybridSPE®-Phospholipid

Conditions

sample/matrix rabbit plasma, unfiltered K2-EDTA, spiked with warfarin at 100 ng/mL (3:1, plasma:1% formic acid in acetonitrile)
SPE tube/cartridge HybridSPE-Phospholipid, 96-well plate (575656-U)
sample addition to each well add 100 µl plasma, followed by a 300 µl of 1% formic acid in acetonitrile, agitate on orbital shaker for 4 minutes
elution collect filtrate and analyze directly
column Astec CHIROBIOTIC(R) V, 10 cm x 4.6 mm I.D., 5 µm particles (11022AST)
column temp. 35 C
mobile phase [A] 0.1% formic acid (pH unadjusted); [B] acetonitrile, 75:25 (A:B)
flow rate 1 ml/min
injection 2 µl
detector ESI+, 100-1000 m/z

Description

Analysis Note The R- and S- forms of warfarin, a commonly prescribed anticoagulant, are cleared by the body via different pathways and have different pharmacokinetic and pharmacodynamic effects. An Astec CHIROBIOTIC V HPLC column provided rapid resolution of the enantiomers under conditions that are compatible with mass spectrometric (MS) detection. By using HybridSPE-Phospholipid for the sample prep, ion-suppressing phospholipids were removed for improved sensitivity and longer column lifetime. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Clinical, Chiral Applications, SPE Applications, Anticoagulants, Clinical, Forensic & Toxicology, anticoagulants, Therapeutic Drug Monitoring
Featured Industry Clinical
Pharmaceutical (small molecule)
Legal Information HybridSPE is a registered trademark of Sigma-Aldrich Co. LLC, Warfarin is a trademark of Wisconsin Alumni Research Foundation, CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC, Astec is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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