LC/MS/MS Analysis of Zopiclone and Metabolites on Ascentis® Express Phenyl-Hexyl

LC/MS/MS Analysis of Zopiclone and Metabolites on Ascentis® Express Phenyl-Hexyl

Conditions

column Ascentis Express Phenyl-Hexyl  10 cm x 2.1 mm, 2.7 µm particles (53336-U)
column temp. 35 °C
mobile phase [A] 13 mM ammonium acetate in water, no pH adjustment; [B] 13 mM ammonium acetate in methanol; (52:48, A:B)
flow rate 0.4 mL/min
pressure 7850 psi (541 bar)
sample 250 ng/mL each in 13 mM ammonium acetate in water, no pH adjustment; [B] 13 mM ammonium acetate in methanol; (50:50 A:B)
injection 1 µL
detector MS, ESI(+), MRM

Description

Analysis Note Zopiclone is a non-benzodiazepine hypnotic sold under the trade names Imovane® and Zimovane and is prescribed for the treatment of insomnia. The Ascentis Express phenyl-Hexyl column gave the best separation of zopiclone and its metabolites of the column chemistries tested. Ultra high purity LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Benzodiazepines
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, Imovane is a registered trademark of Sanofi-Aventis
Other Notes MRM Transitions
1  : 375.11 > 111.92      N-Desmethylzopiclone
2  : 375.11 > 217.00       N-Desmethylzopiclone
3  : 375.11 > 244.98       N-Desmethylzopiclone
4  : 389.08 > 111.92       Zopiclone           
5  : 389.08 > 217.00       Zopiclone           
6  : 389.08 > 244.97       Zopiclone         
7  : 405.13 > 58.01         Zopiclone-N-oxide   
8  : 405.13 > 143.29       Zopiclone-N-oxide   
9  : 405.13 > 245.03      Zopiclone-N-oxide
suitability application for LC-MS

Materials

     
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