LC/MS (NARP-LC-APCI(+)-MS) Analysis of Triacylglycerols in Sparus aurata Fractions 3, 4, and 5 Isolated by 1D Silver Ion on Ascentis® Express C18, Mass Scan Number 14,174 (35.43 min)

LC/MS (NARP-LC-APCI(+)-MS) Analysis of Triacylglycerols in Sparus aurata Fractions 3, 4, and 5 Isolated by 1D Silver Ion on Ascentis® Express C18, Mass Scan Number 14,174 (35.43 min)

Conditions

column Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 µm particles (53829-U)
mobile phase [A] acetonitrile; [B] 2-propanol (IPA)
gradient 0 to 70% B in 50 min; held at 70% B for 5 min; to 0% B in 1 min
flow rate 1 ml/min
sample fish tissue extracted by Folch method, 1D (Ag+ion) dried fractions dissolved in 200 µl of acetone
injection 5 µl
detector MS, APCI(+), mass range 400-1100 m/z

Description

Analysis Note Data provided by Marco Beccaria (1) and Luigi Mondello (1,2) of (1) Chromaleont s.r.l., c/o “Scienze del Farmaco e Prodotti per la Salute” Department, University of Messina, viale Annunziata, 98168 Messina, Italy or (2) “Scienze del Farmaco e Prodotti per la Salute” Department, University of Messina, viale Annunziata, 98168 Messina, Italy.

Triacylglycerols extracted from fish tissue were analyzed by an off-line combination of silver-ion liquid chromatography using a Nucleosil SA column and non-aqueous reversed-phase liquid chromatography (NARP) using an Ascentis Express C18 column. Shown here is the second dimension of the Folch-extracted sample focussing on a particular region of the chromatogram. PN is the carbon number (CN) minus twice the double bond number (DBN).
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
Related Links