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Loading Capacity of RNaseA on Proteomix® SCX-NP3

Loading Capacity of RNaseA on Proteomix® SCX-NP3

Conditions

column Proteomix SCX-NP3, 5 cm x 4.6 mm I.D., 3 µm particles (Z777151)
column temp. 25 C
mobile phase 10 mM phosphate buffer, pH 6.0
gradient 0-1.0M NaCl (20 min)
flow rate 1.0 ml/min
injection 100 µl
detector UV, 280 nm

Description

Analysis Note Courtesy of Miyako Kawakatsu, M&S Instruments, Inc., This application note examines the loading capacity of Ribonuclease A on a Proteomix SCX-NP3 column. Sample sizes ranging from 50 μg to 5 mg were examined in this analysis.
Categories Analytical Chromatography, Stiµlants
Featured Industry Life Science and Biopharma
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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