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NP-HPLC with Fluorimetric Detector of Tocopherols in Extra Virgin Olive Oil Samples on Ascentis® Si

NP-HPLC with Fluorimetric Detector of Tocopherols in Extra Virgin Olive Oil Samples on Ascentis® Si

Conditions

column Ascentis Si, 10 cm x 1.0 mm I.D., 3 µm particles (581520-U)
mobile phase [A] hexane; [B] isopropanol; (99:1; A:B)
flow rate 50 µL/min
detector fluorescence, ex: 290 nm, em: 330 nm

Description

Analysis Note Sample preparation:

Extra virgin olive oil
10 mg diluted in 1 mL of n-hexane

Extra virgin olive oil by-products

100 g of each sample was extracted with 100 mL of n-hexane 3 times, n-hexane fractions were collected and brought to dryness in a rotary evaporator, 10 mg of the residue was diluted in 1 mL of n-hexane prior to analysis
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability suitable for HPLC

Materials

     
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