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RP-HPLC Analysis of Benzodiazepines on Ascentis® Express Biphenyl

RP-HPLC Analysis of Benzodiazepines on Ascentis® Express Biphenyl


column Ascentis Express Biphenyl, 10 cm x 3.0 mm I.D., 2.7 µm particles (64066-U)
column temp. 35 C
mobile phase [A] Water; [B] acetonitrile; (70:30, A:B)
flow rate 1.0 ml/min
pressure 5294 psi (365 bar)
sample Benzodiazepine µlticomponent Mixture-8 diluted to 50ug/mL in 50:50 Water:Methanol
injection 1.0 µl
detector UV, 254 nm


Analysis Note This analysis demonstrates the applicability of the Ascentis Express Biphenyl stationary phase for the analysis of benzodiazepines, a group of psychoactive drugs that act as GABA agonists. The stationary phase provides alternative selectivity and improved resolution to existing phenyl chemistires.
Categories Analytical Chromatography, Benzodiazepines
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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