RP-HPLC Analysis of Substituted Phenethylamine Psychedelics on Ascentis® Express 2.7 μm RP-Amide

RP-HPLC Analysis of Substituted Phenethylamine Psychedelics on Ascentis® Express 2.7 μm RP-Amide

Conditions

column Ascentis Express RP-Amide 10cm x 2.1 mm I.D., 2.7 µm particles (53913-U)
column temp. 35 °C
mobile phase [A] 20 mM Ammonium acetate pH 4.0 with Acetic  Acid; [B] ACN; (85:15, A:B)
gradient 15% B for 2 min; 15 to 30% B in 6 min, held at 30% B for 4 min
flow rate 0.6 mL/min
pressure 4757 psi (328 bar)
sample 25C-NBOMe HCl, 25B-NBOMe HCl, 25I-NBF HCl, 25INBMD HCl, 25I-NBOME HCl, 20 µg/mL in 90:10 mobile phase A:mobile phase B
injection 5 µL
detector UV, 280 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis Express RP-Amide for the HPLC separation of substituted phenethylamine psychedelics (Bath Salts). This application shows the use of UV detection for this set of compounds.
Categories Analytical Chromatography, Drugs of Abuse
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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