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Typical Desalting Chromatograms on TSKgel® BioAssist® DS HPLC Columns, Increasing Sample Injection Volume

Typical Desalting Chromatograms on TSKgel® BioAssist® DS HPLC Columns, Increasing Sample Injection Volume

Conditions

column TSKgel BioAssist DS, 15 cm x 10 mm I.D., 13 µm particles (821464)
column temp. 4 C
mobile phase 50 mM sodium phosphate buffer, pH 6.5
flow rate 3 mL/min50 mM sodium phosphate buffer, pH 6.5
sample 10 mg/mL ovalbumin in 0.5 M NaCl and 25 mM sodium phosphate buffer, pH 6.5
injection 10 µl
detector UV, 280 nm and refractive index (RI)

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information BioAssist is a registered trademark of Tosoh Corporation, TSKgel is a registered trademark of Tosoh Corporation
Other Notes Data provided by Tosoh Bioscience LLC.
suitability application for HPLC

Materials

     
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