UHPLC Analysis of a Protein Standard on TSKgel® UP-SW3000: Speed Advantage

UHPLC Analysis of a Protein Standard on TSKgel® UP-SW3000: Speed Advantage

Conditions

column TSKgel SuperSW3000, 30 cm x 4.6 mm I.D., 4.0 µm particles (818675)
column TSKgel UP-SW3000, 15 cm x 4.6 mm I.D., 2.0 µm particles
column temp. 25 C
mobile phase 0.2 M sodium phosphate, dibasic, pH 7.0 with phosphoric acid
flow rate 0.3 ml/min
pressure 1280SuperSW3000 (88 bar)
pressure 1900UP-SW3000 (131 bar)
sample protein standard mix
injection 2 µl (SuperSW3000)
injection 1 µl (UP-SW3000)
detector UV, 215 nm

Description

Analysis Note A protein standard was chromatographed on a TSKgel® UP-SW3000, 15 cm x 4.6 mm I.D., 2.0 μm and TSKgel SuperSW3000, 30 cm x 4.6 mm I.D., 4.0 μm to compare the speed of analysis and performance on shorter column length. In comparison to the SuperSW3000, 4 μm column, the UP-SW3000, 2.0 μm column provides greater than or equal the efficiency, in half the time.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information TSKgel is a registered trademark of Tosoh Corporation
suitability application for UHPLC

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