UHPLC Analysis of Aflatoxins on Titan™ C18 with TFA Derivatization and Fluoresence Detection

UHPLC Analysis of Aflatoxins on Titan™ C18 with TFA Derivatization and Fluoresence Detection

Conditions

sample preparation 200 µL of standard solution of aflatoxins was mixed with 300 µL of derivatization solution (7:1:2 water:acetic acid:trifluoroacetic acid). (The mixture was kept at 65 °C for 25 minutes, then cooled down to room temperature. 550 µL of water was added.)
column Titan C18, 10 cm x 2.1 mm I.D., 1.9 µm particles (577124-U)
column temp. 35 °C
mobile phase [A] 5% acetonitrile in water; [B] acetonitrile.
gradient 10 to 30% B in 3 min; to 100% B in 2 min; held at 100% B for 2 min; to 10% B in 0.5 min; held at 10% B for 2.5 min
flow rate 0.4 mL/min
sample standard solution of aflatoxins contained 1.2 ng/mL each of afltoxin B1 and aflatoxin G1, and 0.3 ng/mL each of aflatoxin B2 and afltoxin G2 in acetonitrile:water (84:16)
injection 20 µL
detector Fluorescence, 360/440 Ex/Em

Description

Analysis Note An optimized method for sensitive detection of low levels of aflatoxins using a TFA-derivatization procedure is shown here.
Categories Analytical Chromatography, Mycotoxins
Featured Industry Food and Beverages
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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