UHPLC Analysis of Antifungals on Ascentis® Express C18

UHPLC Analysis of Antifungals on Ascentis® Express C18

Conditions

column Ascentis Express C18 5 cm x 3.0 mm I.D., 2.7 µm particles (53811-U)
column temp. 35 °C
mobile phase [A] 5 mM ammonium acetate pH 6.8 unadjusted in 80:20, water:acetonitrile; [B] 5 mM ammonium acetate pH 6.8 unadjusted in 5:95, water:acetonitrile;
gradient 0% B; to 100% B in 3 min; 100% B held for 0.6 min
flow rate 1.0 mL/min
pressure 2790 psi (192 bar)
sample 6 antifungal 50 µg/mL in 70:30, water:methanol
injection 1 µL
detector UV, 205 nm

Description

Analysis Note The extremely rapid (~3 minutes) resolution of six antifungal compounds on an Ascentis Express C18 column is shown in this application. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Antifungals
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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